AUTHOR=Csepregi Anna Beáta , Papp Eszter , Adamik Imola , Csernák Erzsébet , Engi Helga , Küronya Zsófia , Soós Edina , Melegh Zsombor , Tóth Erika TITLE=Impact of comprehensive genomic profiling on the diagnosis and clinical management of malignant mesenchymal tumours JOURNAL=Pathology and Oncology Research VOLUME=31 YEAR=2025 URL=https://www.por-journal.com/journals/pathology-and-oncology-research/articles/10.3389/pore.2025.1612065 DOI=10.3389/pore.2025.1612065 ISSN=1532-2807 ABSTRACT=
Comprehensive genomic profiling (CGP) is becoming an increasingly important tool in the clinical management of different tumours, but there is still very limited data available on its usefulness from a therapeutic point of view in mesenchymal tumours. Between January 2022 and September 2024, we performed CGP analysis with means of Oncomine Comprehensive Assay Plus (OCAplus) on 94 malignant mesenchymal tumours. The analysis covered more than 500 unique genes for single-gene and multigene biomarker insights, including tumour mutational burden (TMB) and homologous recombination deficiency (HRD). Genomic DNA and total RNA were extracted from formalin-fixed paraffin-embedded tissue blocks. Twenty-four out of 94 patients (25.5%) had potentially actionable alterations: 17 (18%) had specific genetic alterations suitable for targeted therapies, 4 (4.2%) had a high TMB (>10 mut/Mb), and 5 (5.3%) had a high HRD score >15). One additional patient had