AUTHOR=Jing Qin , jun Xie F., hui Li C., Na Han , yang Lu H.

TITLE=MYCL1 Amplification and Expression of L-Myc and c-Myc in Surgically Resected Small-Cell Lung Carcinoma

JOURNAL=Pathology and Oncology Research

VOLUME=27

YEAR=2021

URL=https://www.por-journal.com/journals/pathology-and-oncology-research/articles/10.3389/pore.2021.1609775

DOI=10.3389/pore.2021.1609775

ISSN=1532-2807

ABSTRACT=<p><bold>Purpose:</bold> The Myc family, especially <italic>C-MYC</italic> and <italic>MYCL1</italic>, has been found involved in small-cell lung carcinoma (SCLC). Identification of the frequency of C-MYC and MYCL1 expression among SCLC patients may help to identify potential targets for therapeutic intervention. Our aim was to detect <italic>MYCL1</italic> amplification, L-Myc and c-Myc expression, and investigate clinicopathological characteristics and survival status in patients with surgically resected SCLC.</p><p><bold>Methods:</bold><italic>MYCL1</italic> amplification was detected using fluorescence <italic>in situ</italic> hybridization (FISH), while L-Myc and c-Myc protein expressions were determined using immunohistochemistry (IHC) in the primary tumors of 46 resected SCLC patients.</p><p><bold>Results:</bold> Among the 46 evaluated specimens, <italic>MYCL1</italic> amplification was identified in 3/46 cases (6.5%). One of the positive cases was <italic>MYCL1</italic> gene amplification combined with fusion. 3/46 (6.5%) was positive for L-myc protein expression, and 4/46 (8.7%) was positive for c-Myc protein expression.</p><p><bold>Conclusion:</bold> Our study firstly multidimensional explored the expression of <italic>MYCL1</italic> amplification, L-Myc and c-Myc protein and investigated clinicopathological characteristics and survival status in patients with surgically resected SCLC, which makes a contribution to subsequent research and therapeutic strategies.</p>